Coding

Part:BBa_K642002

Designed by: Wilson Lam, Matthew Orton   Group: iGEM11_uOttawa   (2011-09-22)

cI repressor tagged with yBFP

This is the cI repressor from phage lambda tagged with yeast codon optimized BFP. cI will bind to operators already present in the registry: (BBa_R0051 & BBa_K105021). BFP is a monomeric fluorescent protein that has an excitation peak of 399 nm and an emission peak of 465 nm (1). It was yeast codon optimized through DNA synthesis for the purpose of expressing in S. cerevisiae tagged to various repressors and activators.

References

(1) Subach, O. M., I. S. Gundorov, et al. (2008). "Conversion of red fluorescent protein into a bright blue probe." Chem Biol 15(10): 1116-24.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 711
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/acI repressor tagged with yBFP